Dako Autostainer Plus Manuel d'utilisateur

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Dako Autostainer Plus | User Guide 1
Dako Autostainer Plus | User Guide
Document Number 0003107
Revision D
March 2007
For In Vitro Diagnostic Use
UL and cUL
Dako Colorado, Inc.
4850 Innovation Drive
Ft. Collins, CO 80525 USA
970-226-2200
Vue de la page 0
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Résumé du contenu

Page 1 - UL and cUL

Dako Autostainer Plus | User Guide 1 Dako Autostainer Plus | User Guide Document Number 0003107 Revision D March 2007 For In V

Page 2

10 Dako Autostainer Plus | User Guide Data Management and Report Generation The Dako Autostainer Plus is designed to track a variety of data. It

Page 3 - Contact Dako

100 Dako Autostainer Plus | User Guide NOTE: Do not use bleach in the Dako Autostainer Plus. Bleach may react with other chemicals and create toxi

Page 4

Dako Autostainer Plus | User Guide 101 Slide Rack Cleaning Dako recommends additional cleaning for slide racks. 1. Soak the slide racks overn

Page 5 - Table of Contents

102 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 6

Dako Autostainer Plus | User Guide 103 Section 18 | Reagent Tracking Reagent Tracking allows you to monitor the total volume of each reagent

Page 7

104 Dako Autostainer Plus | User Guide Remove Reagent from the Reagent Tracking Log Reagents that are outdated or not critical to tracking can be

Page 8

Dako Autostainer Plus | User Guide 105 Section 19 | Wash Buffers Wash buffers are required for all immunohistochemical staining procedures.

Page 9 - Section 1

106 Dako Autostainer Plus | User Guide 4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05% Tween 20. Five Liter

Page 10 - Slide and Reagent Labels

Dako Autostainer Plus | User Guide 107 Materials Required 1. Clean carboy, flask or beaker graduated to 1L 2. Deionized water 3. Magnetic sti

Page 11

108 Dako Autostainer Plus | User Guide Shelf Life and Storage TBS solutions should be stored at 2-8?C to inhibit bacterial growth. TBS solutions t

Page 12

Dako Autostainer Plus | User Guide 109 Shelf Life and Storage PBS solutions should be stored at 2-8° C to inhibit bacterial growth. PBS solution

Page 13 - Software Specifications

Dako Autostainer Plus | User Guide 11 Section 2 | System Specifications The Dako Autostainer Plus is a combination of hardware and software

Page 14

110 Dako Autostainer Plus | User Guide 4. Once pH 7.0 is reached, add 0.5 mL of Tween 20 and mix using a magnetic stir plate. 5. Adjust the final

Page 15 - Recommendations

Dako Autostainer Plus | User Guide 111 Section 20 | Troubleshooting Symptom Possible Causes Solutions Autostainer Plus will not turn on (t

Page 16

112 Dako Autostainer Plus | User Guide Symptom Possible Causes Solutions Buffer or DI Water does not flow out during a run or a prime. Autostaine

Page 17

Dako Autostainer Plus | User Guide 113 Symptom Possible Causes Solutions Reagent probe is not dispensing reagent. Insufficient reagent in reage

Page 18

114 Dako Autostainer Plus | User Guide Symptom Possible Causes Solutions No Staining Insufficient reagent in reagent vial. Reagents

Page 19

Dako Autostainer Plus | User Guide 115 Symptom Possible Causes Solutions Error occurs during printing. Labels/ribbon has run out. Incorre

Page 20 - File Menu

116 Dako Autostainer Plus | User Guide Symptom Possible Causes Solutions User is unable to access many of the functions in the Initialize section

Page 21

Dako Autostainer Plus | User Guide 117 Symptom Possible Causes Solutions Monitor will not turn on or goes blank intermittently (power LED is no

Page 22 - Command Buttons

118 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 23 - Software

Dako Autostainer Plus | User Guide 119 Section 21 | Warnings and Symbols Keep the cover closed during operation. The robotic arm will move

Page 24

12 Dako Autostainer Plus | User Guide Slide Rack is a slide carrier holding 12 microscope slides in a horizontal position. The instrument holds a

Page 25

120 Dako Autostainer Plus | User Guide Do not relocate the Dako Autostainer Plus System within your facility Do not relocate the Dako Autostainer P

Page 26

Dako Autostainer Plus | User Guide 121 Section 22 | General Limitations Immunohistochemistry is a multi-step diagnostic process that require

Page 27 - Entering Staff Information

Dako Autostainer Plus | User Guide 13 Hardware Specifications Dako Autostainer Plus Dimensions 1.02m W x 0.69 m D x 0.61 m H 40” W x 27” D x

Page 28 - Editing User Information

14 Dako Autostainer Plus | User Guide Accessories included with the Dako Autostainer Plus Slide Racks 4 non-disposable slide racks with a 12-sli

Page 29 - Adding a New Doctor

Dako Autostainer Plus | User Guide 15 Section 3 | Installation Requirements For installation of the Dako Autostainer Plus, the following req

Page 30

16 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 31 - Cleaning Schedule

Dako Autostainer Plus | User Guide 17 Section 4 | Typical Operation A few “off instrument” preparations will make the staining run more time

Page 32 - Slide Labels with Bar Codes

18 Dako Autostainer Plus | User Guide Selecting the UNPRINTED LABELS button will print all labels that were not printed through the PRINT feature a

Page 33

Dako Autostainer Plus | User Guide 19 Section 5 | Programming Overview The Dako Autostainer Plus is a computer controlled system. The PROGRA

Page 34 - Reagent Labels

2 Dako Autostainer Plus | User Guide Dako Autostainer Plus Universal Staining System Dako Autostainer Plus Information Dako Autostainer Model:

Page 35 - Communication Port

20 Dako Autostainer Plus | User Guide Menu Bar Options Slides Function The Slides option may be used as an alternative to expedite programming by

Page 36 - Program Grid

Dako Autostainer Plus | User Guide 21 Edit Lists Menu The Edit Lists menu can be accessed by moving the mouse pointer to Edit

Page 37 - Custom Options

22 Dako Autostainer Plus | User Guide Copy Function The Copy function can be accessed by moving the mouse pointer to Copy on the menu bar a

Page 38

Dako Autostainer Plus | User Guide 23 Section 6 | Accessing the Dako Autostainer Plus Software The SIGN IN screen is the first screen that a

Page 39 - Adding Slides

24 Dako Autostainer Plus | User Guide If the accessing operator is assigned a middle or low security level, the INITIALIZE button is repl

Page 40 - The SLIDE INFORMATION Button

Dako Autostainer Plus | User Guide 25 Section 7 | Initializing the Dako Autostainer Plus The INITIALIZE screen is used to establish and upda

Page 41

26 Dako Autostainer Plus | User Guide The buttons on the INITIALIZE screen and their respective functions are: Entering

Page 42

Dako Autostainer Plus | User Guide 27 7. Type in serial number, name, or unique identifier, for your Autostainer Plus and press ENTER or TAB. 8.

Page 43 - Deleting a Block ID

28 Dako Autostainer Plus | User Guide 5. Type in the same password again and press ENTER. The High security level option becomes highlighted. If a

Page 44 - Slide Rearrangement

Dako Autostainer Plus | User Guide 29 Deleting User Information 1. Select INITIALIZE on the MAIN MENU screen. The INITIALIZE screen is displaye

Page 45

Dako Autostainer Plus | User Guide 3 Contact Dako Corporate Headquarters Denmark +45 44 85 95 00 Fax 44 85 95 95 www.dako.com Distr

Page 46 - Standard Rinse Replacement

30 Dako Autostainer Plus | User Guide Deleting Doctor Information 1. Select INITIALIZE on the MAIN MENU screen. The INITIALIZE screen is displaye

Page 47 - Switch (*Swtch)

Dako Autostainer Plus | User Guide 31 Select the CANCEL button to escape without saving. The MAIN MENU screen is displayed. NOTE: The dispense

Page 48 - Saving a Protocol Template

32 Dako Autostainer Plus | User Guide Select the CANCEL button to escape without saving. The MAIN MENU screen is displayed. NOTE: This number can

Page 49 - Deleting a Protocol Template

Dako Autostainer Plus | User Guide 33 3. Select the desired option for the field. The list box disappears and the selected option is displayed i

Page 50 - Protocol

34 Dako Autostainer Plus | User Guide static information, highlight the text in the desired field and type the text in the text box. Press ENTER. 4

Page 51 - Step in a Protocol

Dako Autostainer Plus | User Guide 35 Label Adjustment To check the position of the text on the slide or reagent label and to generate a test la

Page 52

36 Dako Autostainer Plus | User Guide NOTE: The communication port for the printer is identified using the Port box on the DESIGN SLIDE LABEL scr

Page 53

Dako Autostainer Plus | User Guide 37 NOTE: Selecting the Comment item creates a column for data entry on the Programming Grid printout. This c

Page 54 - Defining Primary Antibodies

38 Dako Autostainer Plus | User Guide NOTE: The Delayed Start Water Rinse options cannot be selected while the No Pre-Rinse option is selected. A

Page 55 - Negative Controls

Dako Autostainer Plus | User Guide 39 Section 8 | Entering Slide Information Slides can be programmed from the PROGRAMMING GRID either throu

Page 56 - Defining Specific Rinses

4 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 57 - Auto Programming

40 Dako Autostainer Plus | User Guide Deleting a Slide When using the Slides option to program a staining run, a slide can be deleted if there is

Page 58

Dako Autostainer Plus | User Guide 41 Entering Slide Information Through the SLIDE INFORMATION Button 1. Select the PROGRAM button on the MAIN

Page 59 - Copy/Paste

42 Dako Autostainer Plus | User Guide NOTE: A maximum number of 48 slides can be entered for each staining run. If the total number of slides prog

Page 60 - Editing a Protocol Step

Dako Autostainer Plus | User Guide 43 Deleting a Case # This method deletes all slides associated with the selected slide identification’s case.

Page 61 - Printing Options

44 Dako Autostainer Plus | User Guide Deleting a Specific Slide This method deletes one slide at a time. 1. On the PROGRAMMING GRID select the sl

Page 62

Dako Autostainer Plus | User Guide 45 Section 9 | Designing a Protocol Protocol templates are defined as a sequential series of steps requir

Page 63 - The Main Grid Report

46 Dako Autostainer Plus | User Guide Protocol Elements The Protocol Elements are categories of reagents used to build the Protocol Outline in th

Page 64 - Viewing Programmed Slides

Dako Autostainer Plus | User Guide 47 Substrate-Batch This step splits the staining run into two distinct operations. The first includes all step

Page 65

48 Dako Autostainer Plus | User Guide 5. Additional steps can be inserted in the Protocol Outline by highlighting a step in this column and then c

Page 66 - ALL SLIDES Tool

Dako Autostainer Plus | User Guide 49 Deleting a Protocol Template NOTE: Low level security does not allow access to deleting Protocol Template

Page 67 - Printing a Slide Layout Map

Dako Autostainer Plus | User Guide 5 Table of Contents Section 1 | Introduction ...

Page 68

50 Dako Autostainer Plus | User Guide 2. Select the GET TEMPLATE button. The GET TEMPLATE screen is displayed with the cursor in the File Name box

Page 69

Dako Autostainer Plus | User Guide 51 2. Select the desired reagent dispense volume by clicking on the raised diamond next to the appropriate v

Page 70 - Adding Primary Antibodies

52 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 71

Dako Autostainer Plus | User Guide 53 Section 10 | Programming Slides The Dako Autostainer Plus can process a maximum of 48 microscope sli

Page 72 - Editing Reagent Lists

54 Dako Autostainer Plus | User Guide Defining Primary Antibodies Each primary antibody reagent is programmed individually and will appear in the

Page 73

Dako Autostainer Plus | User Guide 55 3. To insert a matching positive control, click on the POS.CTL button. One slide will be added to the PROG

Page 74 - Printing Reagent Labels

56 Dako Autostainer Plus | User Guide 4. A compatibility code must be entered. Enter the correct compatibility code for the antibody (see Compati

Page 75 - Compatibility Check

Dako Autostainer Plus | User Guide 57 selection: rinse buffer, rinse (DI) water, blow, or none (for details of each option, see Designing a Prot

Page 76

58 Dako Autostainer Plus | User Guide row is high-lighted. Release the mouse button and the Save Auto Program window is displayed. NOTE: An Auto

Page 77 - Reagent Layout Map Screen

Dako Autostainer Plus | User Guide 59 4. Select the desired Auto program file name. The Auto program is inserted where indicated in step 3 above

Page 78 - Missing Reagent Error

6 Dako Autostainer Plus | User Guide The Options Screen ...

Page 79 - Partial Reagent Application

60 Dako Autostainer Plus | User Guide Copy/Paste Entire Rows 1. Select Copy from the menu bar on the PROGRAMMING GRID. The word Copy changes to Se

Page 80

Dako Autostainer Plus | User Guide 61 Editing a reagent in a staining step for one run only: 1. To edit a reagent in a staining step or to use

Page 81 - Loading Microscope Slides

62 Dako Autostainer Plus | User Guide 1. From the PROGRAMMING GRID, select the PRINT button. The WHICH REPORT? screen appears. 2. Select the SLID

Page 82 - Scanning Slides

Dako Autostainer Plus | User Guide 63 Printing reagent labels not from a program. 1. From the PROGRAMMING GRID, select the PRINT button. The WHI

Page 83 - Section 14

64 Dako Autostainer Plus | User Guide The IHC Report can be configured to print in one of the following formats: • The One case/page option print

Page 84

Dako Autostainer Plus | User Guide 65 Each cell on the screen represents a microscope slide. Cells contain the position number, case number and

Page 85 - Delaying the Start of a Run

66 Dako Autostainer Plus | User Guide adjusting reagent application amounts. These functions may be performed in the PROGRAMMING GRID, using the AL

Page 86 - Adding Priority Slides

Dako Autostainer Plus | User Guide 67 NOTE: The INDIVIDUAL SLIDE TOOL functions identically to the ALL SLIDES TOOL except the user is only able

Page 87

68 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 88

Dako Autostainer Plus | User Guide 69 Section 11 | Reagent Management Reagent lists are defined using the commands on the Edit lists menu on

Page 89

Dako Autostainer Plus | User Guide 7 Replacing a reagent with skip step (no reagent added to slide): ... 60 Editi

Page 90

70 Dako Autostainer Plus | User Guide 5. Enter the correct compatibility code and press TAB or ENTER. Assigning compatibility information to reage

Page 91

Dako Autostainer Plus | User Guide 71 3. Type the full name of the antibody you want to add to the current list and press TAB or ENTER. The soft

Page 92

72 Dako Autostainer Plus | User Guide NOTE: Manual Step setting can also be changes from the Programming Grid, see Programming Slides, section 10.

Page 93

Dako Autostainer Plus | User Guide 73 Deleting Reagents from Reagent Lists 1. From the PROGRAMMING GRID select Edit lists on the menu bar. The

Page 94 - Printing a Temporary Run Log

74 Dako Autostainer Plus | User Guide Printing Reagent Labels Printing reagent labels from a programmed run. 1. Program or retrieve a previously s

Page 95 - Immunohistochemical Report

Dako Autostainer Plus | User Guide 75 Compatibility Check A compatibility check has been built into the Dako Autostainer Plus software to minim

Page 96

76 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 97

Dako Autostainer Plus | User Guide 77 Section 12 | Loading Reagents After programming is completed the Dako Autostainer Plus calculates the

Page 98

78 Dako Autostainer Plus | User Guide The buttons and their functions are: 1. The reagent volume displayed in the REAGENT LAYOUT MAP screen inclu

Page 99 - Automated Cleaning Cycle

Dako Autostainer Plus | User Guide 79 reagent vial. If sufficient volume of a reagent is not available, the Autostainer Plus will provide a list

Page 100 - Reservoir Cleaning

8 Dako Autostainer Plus | User Guide Printing a Run Log...

Page 101 - Backing Up Files

80 Dako Autostainer Plus | User Guide 2. A 60 second count begins to count down and an alarm sounds. 3. If the user does not notice the MORE REA

Page 102

Dako Autostainer Plus | User Guide 81 Section 13 | Loading and Scanning Slides After programming is completed and the reagents have been loa

Page 103

82 Dako Autostainer Plus | User Guide Scanning Slides 1. Select SCAN SLIDES from the LOAD AND SCAN SLIDES screen. 2. The system will ask you to

Page 104

Dako Autostainer Plus | User Guide 83 Section 14 | Starting a Run After the specimen-containing slides and reagent vials have been loaded an

Page 105 - Section 19

84 Dako Autostainer Plus | User Guide The Start Time is listed as +0:01 and the current time. The Finish Time is listed as the +HH:MM required to

Page 106 - Intended Use

Dako Autostainer Plus | User Guide 85 The current step, total and current time are always displayed in blue at the bottom of the Run Log. NOTE:

Page 107 - Reagent Preparation

86 Dako Autostainer Plus | User Guide NOTE: The “01” hardware version of the Dako Autostainer Plus does not have an additional reservoir for deion

Page 108

Dako Autostainer Plus | User Guide 87 NOTE: The Priority Slides feature is not available until the initial Pre-Rinse is complete. 2. After the

Page 109 - Phosphate Buffered Saline

88 Dako Autostainer Plus | User Guide Select the NO button to combine the priority slides with the original slide run and complete the staining of

Page 110

Dako Autostainer Plus | User Guide 89 Select the NO button for the “Ready to Unload Priority Slides” prompt if the priority slides should remain

Page 111 - Section 20

Dako Autostainer Plus | User Guide 9 Section 1 | Introduction The Dako Autostainer Plus System is an automated slide processing system compa

Page 112

90 Dako Autostainer Plus | User Guide Select the NO button to return to the RUN LOG screen to continue the original run. NOTE: The time display bu

Page 113

Dako Autostainer Plus | User Guide 91 Delaying the Start of a Run using Substrate-Batch Batch Time indicates when to load the freshly prepared s

Page 114

92 Dako Autostainer Plus | User Guide This page is intentionally blank.

Page 115

Dako Autostainer Plus | User Guide 93 Section 15 | Completing a Staining Run During a staining run the RUN LOG screen is displayed and updat

Page 116

94 Dako Autostainer Plus | User Guide 3. Position the slide rack for unloading slides by picking up the slide rack, tilting it at a 30° angle away

Page 117

Dako Autostainer Plus | User Guide 95 4. Position the mouse pointer over the Name heading and press the mouse button. All the files will be list

Page 118

96 Dako Autostainer Plus | User Guide • the No page break option prints all slides for a run in a continuous print out The IHC Report groups all

Page 119

Dako Autostainer Plus | User Guide 97 Section 16 | Programming During Staining Run This feature allows the user to program subsequent runs w

Page 120 - Symbol Definitions

98 Dako Autostainer Plus | User Guide Reviewing the Current Program during a Staining Run This feature allows the user to review the PROGRAMMING

Page 121

Dako Autostainer Plus | User Guide 99 Section 17 | System Maintenance It is recommended that the Dako Autostainer Plus be cleaned after 150

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