Dako Autostainer Plus | User Guide 1 Dako Autostainer Plus | User Guide Document Number 0003107 Revision D March 2007 For In V
10 Dako Autostainer Plus | User Guide Data Management and Report Generation The Dako Autostainer Plus is designed to track a variety of data. It
100 Dako Autostainer Plus | User Guide NOTE: Do not use bleach in the Dako Autostainer Plus. Bleach may react with other chemicals and create toxi
Dako Autostainer Plus | User Guide 101 Slide Rack Cleaning Dako recommends additional cleaning for slide racks. 1. Soak the slide racks overn
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Dako Autostainer Plus | User Guide 103 Section 18 | Reagent Tracking Reagent Tracking allows you to monitor the total volume of each reagent
104 Dako Autostainer Plus | User Guide Remove Reagent from the Reagent Tracking Log Reagents that are outdated or not critical to tracking can be
Dako Autostainer Plus | User Guide 105 Section 19 | Wash Buffers Wash buffers are required for all immunohistochemical staining procedures.
106 Dako Autostainer Plus | User Guide 4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05% Tween 20. Five Liter
Dako Autostainer Plus | User Guide 107 Materials Required 1. Clean carboy, flask or beaker graduated to 1L 2. Deionized water 3. Magnetic sti
108 Dako Autostainer Plus | User Guide Shelf Life and Storage TBS solutions should be stored at 2-8?C to inhibit bacterial growth. TBS solutions t
Dako Autostainer Plus | User Guide 109 Shelf Life and Storage PBS solutions should be stored at 2-8° C to inhibit bacterial growth. PBS solution
Dako Autostainer Plus | User Guide 11 Section 2 | System Specifications The Dako Autostainer Plus is a combination of hardware and software
110 Dako Autostainer Plus | User Guide 4. Once pH 7.0 is reached, add 0.5 mL of Tween 20 and mix using a magnetic stir plate. 5. Adjust the final
Dako Autostainer Plus | User Guide 111 Section 20 | Troubleshooting Symptom Possible Causes Solutions Autostainer Plus will not turn on (t
112 Dako Autostainer Plus | User Guide Symptom Possible Causes Solutions Buffer or DI Water does not flow out during a run or a prime. Autostaine
Dako Autostainer Plus | User Guide 113 Symptom Possible Causes Solutions Reagent probe is not dispensing reagent. Insufficient reagent in reage
114 Dako Autostainer Plus | User Guide Symptom Possible Causes Solutions No Staining Insufficient reagent in reagent vial. Reagents
Dako Autostainer Plus | User Guide 115 Symptom Possible Causes Solutions Error occurs during printing. Labels/ribbon has run out. Incorre
116 Dako Autostainer Plus | User Guide Symptom Possible Causes Solutions User is unable to access many of the functions in the Initialize section
Dako Autostainer Plus | User Guide 117 Symptom Possible Causes Solutions Monitor will not turn on or goes blank intermittently (power LED is no
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Dako Autostainer Plus | User Guide 119 Section 21 | Warnings and Symbols Keep the cover closed during operation. The robotic arm will move
12 Dako Autostainer Plus | User Guide Slide Rack is a slide carrier holding 12 microscope slides in a horizontal position. The instrument holds a
120 Dako Autostainer Plus | User Guide Do not relocate the Dako Autostainer Plus System within your facility Do not relocate the Dako Autostainer P
Dako Autostainer Plus | User Guide 121 Section 22 | General Limitations Immunohistochemistry is a multi-step diagnostic process that require
Dako Autostainer Plus | User Guide 13 Hardware Specifications Dako Autostainer Plus Dimensions 1.02m W x 0.69 m D x 0.61 m H 40” W x 27” D x
14 Dako Autostainer Plus | User Guide Accessories included with the Dako Autostainer Plus Slide Racks 4 non-disposable slide racks with a 12-sli
Dako Autostainer Plus | User Guide 15 Section 3 | Installation Requirements For installation of the Dako Autostainer Plus, the following req
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Dako Autostainer Plus | User Guide 17 Section 4 | Typical Operation A few “off instrument” preparations will make the staining run more time
18 Dako Autostainer Plus | User Guide Selecting the UNPRINTED LABELS button will print all labels that were not printed through the PRINT feature a
Dako Autostainer Plus | User Guide 19 Section 5 | Programming Overview The Dako Autostainer Plus is a computer controlled system. The PROGRA
2 Dako Autostainer Plus | User Guide Dako Autostainer Plus Universal Staining System Dako Autostainer Plus Information Dako Autostainer Model:
20 Dako Autostainer Plus | User Guide Menu Bar Options Slides Function The Slides option may be used as an alternative to expedite programming by
Dako Autostainer Plus | User Guide 21 Edit Lists Menu The Edit Lists menu can be accessed by moving the mouse pointer to Edit
22 Dako Autostainer Plus | User Guide Copy Function The Copy function can be accessed by moving the mouse pointer to Copy on the menu bar a
Dako Autostainer Plus | User Guide 23 Section 6 | Accessing the Dako Autostainer Plus Software The SIGN IN screen is the first screen that a
24 Dako Autostainer Plus | User Guide If the accessing operator is assigned a middle or low security level, the INITIALIZE button is repl
Dako Autostainer Plus | User Guide 25 Section 7 | Initializing the Dako Autostainer Plus The INITIALIZE screen is used to establish and upda
26 Dako Autostainer Plus | User Guide The buttons on the INITIALIZE screen and their respective functions are: Entering
Dako Autostainer Plus | User Guide 27 7. Type in serial number, name, or unique identifier, for your Autostainer Plus and press ENTER or TAB. 8.
28 Dako Autostainer Plus | User Guide 5. Type in the same password again and press ENTER. The High security level option becomes highlighted. If a
Dako Autostainer Plus | User Guide 29 Deleting User Information 1. Select INITIALIZE on the MAIN MENU screen. The INITIALIZE screen is displaye
Dako Autostainer Plus | User Guide 3 Contact Dako Corporate Headquarters Denmark +45 44 85 95 00 Fax 44 85 95 95 www.dako.com Distr
30 Dako Autostainer Plus | User Guide Deleting Doctor Information 1. Select INITIALIZE on the MAIN MENU screen. The INITIALIZE screen is displaye
Dako Autostainer Plus | User Guide 31 Select the CANCEL button to escape without saving. The MAIN MENU screen is displayed. NOTE: The dispense
32 Dako Autostainer Plus | User Guide Select the CANCEL button to escape without saving. The MAIN MENU screen is displayed. NOTE: This number can
Dako Autostainer Plus | User Guide 33 3. Select the desired option for the field. The list box disappears and the selected option is displayed i
34 Dako Autostainer Plus | User Guide static information, highlight the text in the desired field and type the text in the text box. Press ENTER. 4
Dako Autostainer Plus | User Guide 35 Label Adjustment To check the position of the text on the slide or reagent label and to generate a test la
36 Dako Autostainer Plus | User Guide NOTE: The communication port for the printer is identified using the Port box on the DESIGN SLIDE LABEL scr
Dako Autostainer Plus | User Guide 37 NOTE: Selecting the Comment item creates a column for data entry on the Programming Grid printout. This c
38 Dako Autostainer Plus | User Guide NOTE: The Delayed Start Water Rinse options cannot be selected while the No Pre-Rinse option is selected. A
Dako Autostainer Plus | User Guide 39 Section 8 | Entering Slide Information Slides can be programmed from the PROGRAMMING GRID either throu
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40 Dako Autostainer Plus | User Guide Deleting a Slide When using the Slides option to program a staining run, a slide can be deleted if there is
Dako Autostainer Plus | User Guide 41 Entering Slide Information Through the SLIDE INFORMATION Button 1. Select the PROGRAM button on the MAIN
42 Dako Autostainer Plus | User Guide NOTE: A maximum number of 48 slides can be entered for each staining run. If the total number of slides prog
Dako Autostainer Plus | User Guide 43 Deleting a Case # This method deletes all slides associated with the selected slide identification’s case.
44 Dako Autostainer Plus | User Guide Deleting a Specific Slide This method deletes one slide at a time. 1. On the PROGRAMMING GRID select the sl
Dako Autostainer Plus | User Guide 45 Section 9 | Designing a Protocol Protocol templates are defined as a sequential series of steps requir
46 Dako Autostainer Plus | User Guide Protocol Elements The Protocol Elements are categories of reagents used to build the Protocol Outline in th
Dako Autostainer Plus | User Guide 47 Substrate-Batch This step splits the staining run into two distinct operations. The first includes all step
48 Dako Autostainer Plus | User Guide 5. Additional steps can be inserted in the Protocol Outline by highlighting a step in this column and then c
Dako Autostainer Plus | User Guide 49 Deleting a Protocol Template NOTE: Low level security does not allow access to deleting Protocol Template
Dako Autostainer Plus | User Guide 5 Table of Contents Section 1 | Introduction ...
50 Dako Autostainer Plus | User Guide 2. Select the GET TEMPLATE button. The GET TEMPLATE screen is displayed with the cursor in the File Name box
Dako Autostainer Plus | User Guide 51 2. Select the desired reagent dispense volume by clicking on the raised diamond next to the appropriate v
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Dako Autostainer Plus | User Guide 53 Section 10 | Programming Slides The Dako Autostainer Plus can process a maximum of 48 microscope sli
54 Dako Autostainer Plus | User Guide Defining Primary Antibodies Each primary antibody reagent is programmed individually and will appear in the
Dako Autostainer Plus | User Guide 55 3. To insert a matching positive control, click on the POS.CTL button. One slide will be added to the PROG
56 Dako Autostainer Plus | User Guide 4. A compatibility code must be entered. Enter the correct compatibility code for the antibody (see Compati
Dako Autostainer Plus | User Guide 57 selection: rinse buffer, rinse (DI) water, blow, or none (for details of each option, see Designing a Prot
58 Dako Autostainer Plus | User Guide row is high-lighted. Release the mouse button and the Save Auto Program window is displayed. NOTE: An Auto
Dako Autostainer Plus | User Guide 59 4. Select the desired Auto program file name. The Auto program is inserted where indicated in step 3 above
6 Dako Autostainer Plus | User Guide The Options Screen ...
60 Dako Autostainer Plus | User Guide Copy/Paste Entire Rows 1. Select Copy from the menu bar on the PROGRAMMING GRID. The word Copy changes to Se
Dako Autostainer Plus | User Guide 61 Editing a reagent in a staining step for one run only: 1. To edit a reagent in a staining step or to use
62 Dako Autostainer Plus | User Guide 1. From the PROGRAMMING GRID, select the PRINT button. The WHICH REPORT? screen appears. 2. Select the SLID
Dako Autostainer Plus | User Guide 63 Printing reagent labels not from a program. 1. From the PROGRAMMING GRID, select the PRINT button. The WHI
64 Dako Autostainer Plus | User Guide The IHC Report can be configured to print in one of the following formats: • The One case/page option print
Dako Autostainer Plus | User Guide 65 Each cell on the screen represents a microscope slide. Cells contain the position number, case number and
66 Dako Autostainer Plus | User Guide adjusting reagent application amounts. These functions may be performed in the PROGRAMMING GRID, using the AL
Dako Autostainer Plus | User Guide 67 NOTE: The INDIVIDUAL SLIDE TOOL functions identically to the ALL SLIDES TOOL except the user is only able
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Dako Autostainer Plus | User Guide 69 Section 11 | Reagent Management Reagent lists are defined using the commands on the Edit lists menu on
Dako Autostainer Plus | User Guide 7 Replacing a reagent with skip step (no reagent added to slide): ... 60 Editi
70 Dako Autostainer Plus | User Guide 5. Enter the correct compatibility code and press TAB or ENTER. Assigning compatibility information to reage
Dako Autostainer Plus | User Guide 71 3. Type the full name of the antibody you want to add to the current list and press TAB or ENTER. The soft
72 Dako Autostainer Plus | User Guide NOTE: Manual Step setting can also be changes from the Programming Grid, see Programming Slides, section 10.
Dako Autostainer Plus | User Guide 73 Deleting Reagents from Reagent Lists 1. From the PROGRAMMING GRID select Edit lists on the menu bar. The
74 Dako Autostainer Plus | User Guide Printing Reagent Labels Printing reagent labels from a programmed run. 1. Program or retrieve a previously s
Dako Autostainer Plus | User Guide 75 Compatibility Check A compatibility check has been built into the Dako Autostainer Plus software to minim
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Dako Autostainer Plus | User Guide 77 Section 12 | Loading Reagents After programming is completed the Dako Autostainer Plus calculates the
78 Dako Autostainer Plus | User Guide The buttons and their functions are: 1. The reagent volume displayed in the REAGENT LAYOUT MAP screen inclu
Dako Autostainer Plus | User Guide 79 reagent vial. If sufficient volume of a reagent is not available, the Autostainer Plus will provide a list
8 Dako Autostainer Plus | User Guide Printing a Run Log...
80 Dako Autostainer Plus | User Guide 2. A 60 second count begins to count down and an alarm sounds. 3. If the user does not notice the MORE REA
Dako Autostainer Plus | User Guide 81 Section 13 | Loading and Scanning Slides After programming is completed and the reagents have been loa
82 Dako Autostainer Plus | User Guide Scanning Slides 1. Select SCAN SLIDES from the LOAD AND SCAN SLIDES screen. 2. The system will ask you to
Dako Autostainer Plus | User Guide 83 Section 14 | Starting a Run After the specimen-containing slides and reagent vials have been loaded an
84 Dako Autostainer Plus | User Guide The Start Time is listed as +0:01 and the current time. The Finish Time is listed as the +HH:MM required to
Dako Autostainer Plus | User Guide 85 The current step, total and current time are always displayed in blue at the bottom of the Run Log. NOTE:
86 Dako Autostainer Plus | User Guide NOTE: The “01” hardware version of the Dako Autostainer Plus does not have an additional reservoir for deion
Dako Autostainer Plus | User Guide 87 NOTE: The Priority Slides feature is not available until the initial Pre-Rinse is complete. 2. After the
88 Dako Autostainer Plus | User Guide Select the NO button to combine the priority slides with the original slide run and complete the staining of
Dako Autostainer Plus | User Guide 89 Select the NO button for the “Ready to Unload Priority Slides” prompt if the priority slides should remain
Dako Autostainer Plus | User Guide 9 Section 1 | Introduction The Dako Autostainer Plus System is an automated slide processing system compa
90 Dako Autostainer Plus | User Guide Select the NO button to return to the RUN LOG screen to continue the original run. NOTE: The time display bu
Dako Autostainer Plus | User Guide 91 Delaying the Start of a Run using Substrate-Batch Batch Time indicates when to load the freshly prepared s
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Dako Autostainer Plus | User Guide 93 Section 15 | Completing a Staining Run During a staining run the RUN LOG screen is displayed and updat
94 Dako Autostainer Plus | User Guide 3. Position the slide rack for unloading slides by picking up the slide rack, tilting it at a 30° angle away
Dako Autostainer Plus | User Guide 95 4. Position the mouse pointer over the Name heading and press the mouse button. All the files will be list
96 Dako Autostainer Plus | User Guide • the No page break option prints all slides for a run in a continuous print out The IHC Report groups all
Dako Autostainer Plus | User Guide 97 Section 16 | Programming During Staining Run This feature allows the user to program subsequent runs w
98 Dako Autostainer Plus | User Guide Reviewing the Current Program during a Staining Run This feature allows the user to review the PROGRAMMING
Dako Autostainer Plus | User Guide 99 Section 17 | System Maintenance It is recommended that the Dako Autostainer Plus be cleaned after 150
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